Typical ion current recordings
using the alpha-hemolysin nanopore.
The recording at the top shows
the current in the open channel absent DNA or RNA. This
open channel current can remain stable for many hours. The recording
at the
bottom shows
the current in the same channel in the presence of poly C RNA
100mers.
Each downward deflection indicates current impedance as a single
RNA molecule
transits the pore. These experiments were performed in 1M KCl
at 23 °C and 120 mV applied potential using a 10 kHz low-pass
filter. The poly C concentration was about 1 x 10-6 M.
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